Carrot variety nun 85935 cac

ABSTRACT

The disclosure provides a new and distinct hybrid carrot variety NUN 85935 CAC as well as seeds and plants and roots thereof. NUN 85935 CAC is an Imperator carrot variety for the cut and peel market segment and is suitable for growing in the open field.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to U.S. Patent Application No.62/676,616, filed May 25, 2018, which is hereby incorporated byreference in its entirety.

FIELD OF THE DISCLOSURE

The present disclosure relates to the field of plant breeding and, morespecifically, to carrot variety NUN 85935 CAC. The disclosure furtherrelates to vegetative reproductions of NUN 85935 CAC, methods for tissueculture of NUN 85935 CAC and regenerating a plant from such a tissueculture, and also to phenotypic variants of NUN 85935 CAC.

The goal of vegetable breeding is to combine various desirable traits ina single variety or hybrid. Such desirable traits may include greateryield, resistance to diseases, insects or other pests, tolerance to heatand drought, better agronomic quality, higher nutritional value,enhanced growth rate and improved root properties.

BACKGROUND OF THE DISCLOSURE

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same genotype. A plant cross-pollinates if pollen comes to it from aflower of a different genotype. Plants that have been self-pollinatedand selected for (uniform) type over many generations become homozygousat almost all gene loci and produce a uniform population of truebreeding progeny of homozygous plants. A cross between two suchhomozygous plants of different lines produces a uniform population ofhybrid plants that are heterozygous for many gene loci. The extent ofheterozygosity in the hybrid is a function of the genetic distancebetween the parents. Conversely, a cross of two plants each heterozygousat a number of loci produces a segregating population of hybrid plantsthat differ genetically and are not uniform. The resultingnon-uniformity makes performance unpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants to makehybrids, optionally three-way hybrids, and the evaluation of the hybridsresulting from the crosses. Pedigree breeding and recurrent selectionare examples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines are developed byselfing and selection of desired phenotypes. The new plants areevaluated to determine which have commercial potential.

One crop species which has been subject to such breeding programs and isof particular value is the carrot. Carrot (Daucus carota subsp.sativus), is a biennial plant that grows a rosette of leaves in thespring and summer, while building up the stout taproot, which storeslarge amounts of sugars for the plant to flower in the second year. Theflowering stem grows several decimeters (e.g., 60-200 cm) tall, with anumbel of white flowers that produce a fruit called a mericarp.

Carrot is grown as a root vegetable, usually orange in color, thoughpurple, red, white, cream, and yellow varieties exist. It has a crisptexture when fresh. The most commonly eaten part of a carrot is theroot, although the greens are edible as well. It is a domesticated formof the wild carrot Daucus carota, native to Europe and SouthwesternAsia. The domestic carrot has been selectively bred for its greatlyenlarged and more palatable, less woody-textured edible taproot. Carrotsare primarily consumed fresh as snack food, raw vegetable or as saladingredient. Carrots are also popular as cooking vegetable and can befrozen and juiced.

United States is one of the largest carrot producers in the world.Between 1994 and 2014, an average production of 1.4 million pounds ofcarrots were produced in the United States (Food and AgricultureOrganization (FAO)). Carrots are grown year-round in the United Stateswith the highest volume coming from California from December to August.

While breeding efforts to date have provided a number of useful carrotvarieties with beneficial traits, there remains a great need in the artfor new varieties with further improved traits. Such plants wouldbenefit farmers and consumers alike by improving crop yields and/orquality. Some breeding objectives include varying the color, size andshape of the root, disease or pest resistance, yield, suitability tovarious climatic circumstances, and storage properties.

SUMMARY OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure provides for a carrot variety NUN 85935 CAC, productsthereof, and methods of using the same. NUN 85935 CAC is an Imperatorcarrot variety for the cut and peel market segment and is suitable forthe open field.

In an aspect, the disclosure provides a seed of carrot variety NUN 85935CAC, wherein a representative sample of said seed has been depositedunder Accession Number NCIMB ______. The disclosure also provides for aplurality of seeds of NUN 85935 CAC. The carrot seed of NUN 85935 CACmay be provided as an essentially homogeneous population of carrot seed.The population of seed of NUN 85935 CAC may be particularly defined asbeing essentially free from other seed. The seed population may be growninto plants to provide an essentially homogeneous population of carrotplants as described herein.

The disclosure also provides a plant grown from a seed of carrot varietyNUN 85935 CAC and a plant part thereof. In another aspect, thedisclosure provides for a hybrid carrot variety NUN 85935 CAC. Thedisclosure also provides for a progeny of carroty variety NUN 85935 CAC.In another aspect, the disclosure provides a plant or a progenyretaining all or all but one, two or three of the “distinguishingcharacteristics” or all or all but one, two or three of the“morphological and physiological characteristics” of carrot variety NUN85935 CAC, and methods for producing that plant or progeny.

In one aspect, the disclosure provides a plant or a progeny having allthe physiological and morphological characteristics of carrot varietyNUN 85935 CAC when grown under the same environmental conditions. Inanother aspect, the plant or such progeny has all or all but one, two,or three of the physiological and morphological characteristics ofcarrot variety NUN 85935 CAC when measured under the same environmentalconditions and e.g., evaluated at significance levels of 1%, 5% or 10%significance (which can also be expressed as p-value) for quantitativecharacteristics, wherein a representative sample of seed of carrotvariety NUN 85935 CAC has been deposited under Accession Number NCIMB______. In another aspect, the plant or progeny has all or all but oneor three of the physiological and morphological characteristics aslisted in Tables 1 and 2 of carrot variety NUN 85935 CAC, when grownunder the same environmental conditions and e.g., evaluated atsignificance levels of 1%, 5% or 10% significance (which can also beexpressed as a p-value) for quantitative characteristics.

In another aspect, a plant of carrot variety NUN 85935 CAC or progenythereof has 12, 13, or more or all of the distinguishing characteristicsas shown in Table 3: 1) shorter plant top height; 2) thinner plant topneck diameter; 3) shorter blade length minus the petiole; 4) shorterpetiole length; 5) thinner petiole diameter; 6) longer taproot length;7) thinner root shoulder diameter; 8) darker green leaf blade color; 9)more petiole pubescence; 10) smoother root surface; 11) prominent halo;12) darker and more reddish orange below ground root skin color; 13)stronger orange root cross section xylem color; and 14) darker reddishorange root cross section phloem color.

In other aspects, the disclosure provides for a plant part obtained fromcarrot variety NUN 85935 CAC, wherein said plant part is: a root, or apart of a root, a harvested root, a root tip, a fruit, a part of afruit, a leaf, a part of a leaf, pollen, an ovule, a cell, a petiole, ashoot or a part thereof, a stem or a part thereof, a cutting, a seed, apart of a seed, seed coat or another maternal tissue which is part of aseed grown on said varieties, hypocotyl, cotyledon, a pistil, an anther,and a flower or a part thereof. Roots are particularly important plantparts. In another aspect, the plant part obtained from carrot varietyNUN 85935 CAC is a cell, optionally a cell in a cell or tissue culture.That cell may be grown into a plant of carrot variety NUN 85935 CAC.

The disclosure also provides a cell culture of carrot variety NUN 85935CAC and a plant regenerated from carrot variety NUN 85935 CAC, whichplant has all the characteristics of carrot variety NUN 85935 CAC, whengrown under the same environmental conditions, as well as methods forculturing and regenerating carrot variety NUN 85935 CAC. Alternatively,a regenerated plant may have one characteristic that is different fromcarroty variety NUN 85935 CAC.

The disclosure further provides a vegetatively propagated plant ofvariety NUN 85935 CAC is provided having all or all but one, two orthree of the morphological and physiological characteristics of carrotvariety NUN 85935 CAC, when grown under the same environmentalconditions.

The disclosure furthermore provides a carrot root produced on a plantgrown from a seed of NUN 85935 CAC.

In another aspect, the disclosure provides a seed growing or grown on aplant of carrot variety NUN 85935 CAC (i.e., produced after pollinationof the flower of carrot variety NUN 85935 CAC).

Definitions

“Carrot” refers herein to plants of the species Daucus carota. The mostcommonly eaten part of a carrot is the root.

“Cultivated carrot” refers to plants of Daucus carota (e.g., varieties,breeding lines or cultivars of the species D. carota, as well ascrossbreds thereof, or crossbreds with other Daucus carota species),cultivated by humans and having good agronomic characteristics.

“Imperator carrot” refers to long tapered carrots that store well.

The terms “carrot plant designated NUN 85935 CAC”, “NUN 85935 CAC”, “NUN85935”, “NUN 85935 F1”, “85935 CAC” or “carrot 85935” are usedinterchangeably herein and refer to a carrot plant of variety NUN 85935CAC, representative seed has been deposited under Accession Number NCIMB______.

A “seed of NUN 85935 CAC” refers to a carrot seed which can be growninto a plant of carrot variety NUN 85935 CAC, wherein a representativesample of viable seed of carrot variety NUN 85935 CAC has been depositedunder Accession Number NCIMB ______. A seed can be in any stage ofmaturity, for example, a mature, viable seed, or an immature, non-viableseed. A seed comprises an embryo and maternal tissues.

An “embryo of NUN 85935 CAC” refers to an “F1 hybrid embryo” as presentin a seed of carrot variety NUN 85935 CAC, a representative sample ofsaid seed of carrot variety NUN 85935 CAC has been deposited underAccession Number NCIMB ______.

A “seed grown on NUN 85935 CAC” refers to a seed grown on a mature plantof carrot variety NUN 85935 CAC or inside a fruit of NUN 85935 CAC. The“seed grown on NUN 85935 CAC” contains tissues and DNA of the maternalparent, NUN 85935 CAC. The “seed grown on NUN 85935 CAC” contains an F2embryo. When said seed is planted, it grows into a first generationprogeny plant of carrot variety NUN 85935 CAC.

An “essentially homogeneous population of carrot plants” is a populationof plants where at least 97%, 98%, 99% or more of the total populationof plants are plants of carrot variety NUN 85935 CAC.

The phrase “essentially free from other seed” refers to a population ofseed where less than 3%, 2%, 1% or less of the total population of seedis seed that is not a carrot seed or, in another aspect, less than 3%,2%, 1% or less of the total population of seed is seed that is not seedof carrot variety NUN 85935 CAC.

“Tissue culture” or “cell culture” refers to a composition comprisingisolated cells of the same or a different type or a collection of suchcells organized into parts of a plant. Tissue culture of various tissuesof carrot and regeneration of plants therefrom is well known and widelypublished (see, e.g., Arnholdt-Schmitt et al., 1995 Theor Appl Genet(1995) 91:809-815; Larkin and Scowcroft, (1981) Theor. Appl. Genet. 60,197-214). Similarly, the methods of preparing cell cultures are known inthe art.

“USDA descriptors” are the plant variety descriptors described forcarrot in the “Objective description of Variety—Carrot (Daucus carota),”as published by U.S. Department of Agriculture, Agricultural MarketingService, Science and Technology, Plant Variety Protection Office,Beltsville, Md. 20705 and which can be downloaded from the world-wideweb at ams.usda.gov/ underservices/plant-variety-protection/pvpo-c-forms under carrot. “Non-USDAdescriptors” are other descriptors suitable for describing carrot.

“UPOV descriptors” are the plant variety descriptors described forcarrot in the “Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability, TG/49/8 (Geneva 2007, last updated in2015-03-25), as published by UPOV (International Union for theProtection of New Varieties and Plants) and which can be downloaded fromthe world wide web at upov.int/ under edocs/tgdocs/en/tg049.pdf and isherein incorporated by reference in its entirety. Likewise, “UPOVmethods” to determine specific parameters for the characterization ofcarrot are described at upov.int.

“RHS” refers to the Royal Horticultural Society (UK), which publishes anofficial botanical color chart quantitatively identifying colorsaccording to a defined numbering system. The chart may be purchased fromRoyal Horticulture Society Enterprise Ltd RHS Garden; Wisley, Woking;Surrey GU236QB, UK, e.g., the RHS color chart: 2007.

“Plant part” includes any part of a plant, such as a plant organ (e.g.,harvested or non-harvested roots), a plant cell, a plant protoplast, aplant cell tissue culture or a tissue culture from which a whole plantcan be regenerated, a plant cell that is intact in a plant, a clone, amicropropagation, plant callus, a plant cell clump, a plant transplant,a vegetative propagation, a seedling, or a part of a plant (e.g.,harvested tissues or organs), such as a root, or a part of a root, aharvested root, a root tip, a fruit, a harvested fruit, a part of afruit, a leaf, a part of a leaf, pollen, an ovule, an embryo, a petiole,a shoot or a part thereof, a stem or a part thereof, a cutting, a seed,a part of a seed, seed coat or another maternal tissue, hypocotyl,cotyledon, a pistil, an anther, and a flower or parts of any of theseand the like. Seed can be mature or immature. Pollen or ovules may beviable or non-viable. Also, any developmental stage is included, such asseedlings, cuttings prior or after rooting, mature plants or leaves.Alternatively, a plant part may also include a plant seed whichcomprises maternal tissues of carrot variety NUN 85935 CAC, and anembryo having one or two sets of chromosomes derived from the parentplant, e.g., from carrot variety NUN 85935 CAC. Such an embryo comprisestwo sets of chromosomes derived from carrot variety NUN 85935 CAC, if itproduced from self-pollination of said variety, while an embryo derivedfrom cross-fertilization of carrot variety NUN 85935 CAC will compriseonly one set of chromosomes from said variety.

“Reference Variety” for NUN 85935 CAC refers herein to variety PS 1441,a commercial variety from Seminis, which has been planted in a trialtogether with carrot variety NUN 85935 CAC. The characteristics ofcarrot variety NUN 85935 CAC were compared to characteristics of varietyPS 1441 as shown in Tables 1 and 2. The distinguishing characteristicsbetween carrot variety NUN 85935 CAC and the Reference Variety (PS 1441)are shown in Table 3.

“Harvest maturity” refers to the stage at which a carrot root is readyfor harvest or the optimal time to harvest the root for the market, forprocessing or for consumption. In one aspect, harvest maturity is thestage suitable for producing baby carrots.

“Harvested plant material” refers herein to plant parts (e.g., rootsremoved from the soil in which they were growing) which have beencollected for further storage and/or further use.

“Yield” means the total weight of all carrot roots harvested per hectareof a particular line or variety. It is understood that “yield” expressedas weight of all carrots harvested per hectare can be obtained bymultiplying the number of plants per hectare times the “yield perplant”.

“Marketable yield” means the total weight of all marketable carrotroots, especially roots that are not split, damaged or diseased,harvested per hectare of a particular line or variety.

“Refractometer % of soluble solids” refers to the percentage of solublesolids in juice of pureed roots (mainly sugar), as defined by the USDA.It is also expressed as ° Brix and indicates sweetness in the roots ofcarrot. Brix can be measured using a Brix meter (also known asRefractometer).

“Uniform throughout the root” refers to a characteristic such as colorbeing identical throughout the entire plant part (e.g., throughout theroot when it is cut in half).

A plant having “all the physiological and morphological characteristics”of a referred-to-plant means a plant showing the physiological andmorphological characteristics of the referred-to-plant when grown underthe same environmental conditions, preferably in the same experiment;the referred-to-plant can be a plant from which it was derived, e.g.,the progenitor plant, the parent, the recurrent parent, the plant usedfor tissue- or cell culture, etc. A physiological or morphologicalcharacteristic can be a numerical characteristic or a non-numericalcharacteristic. In one aspect, a plant has “all but one, two or three ofthe physiological and morphological characteristics” of areferred-to-plant, or “all the physiological and morphologicalcharacteristics” of Tables 1 and 2 or “all or all but one, two or threeof the physiological and morphological characteristics” of Tables 1 and2.

The physiological and/or morphological characteristics mentioned aboveare commonly evaluated at significance levels of 1%, 5% or 10% if theyare numerical (quantitative), or for having an identical degree (ortype) if not numerical, if measured under the same environmentalconditions. For example, a progeny plant or a Single Locus Convertedplant or a mutated plant of carrot variety NUN 85935 CAC may have one ormore (or all) of the essential physiological and/or morphologicalcharacteristics of said variety listed in Tables 1 and 2, as determinedat the 5% significance level (i.e., p<0.05), when grown under the sameenvironmental conditions.

“Distinguishing characteristics” or “distinguishing morphological and/orphysiological characteristics” refers herein to the characteristicswhich distinguish the new variety from the other carrot varieties, suchas the Reference Variety (i.e., are different), when grown under thesame environmental conditions. The distinguishing characteristicsbetween carrot variety NUN 85935 CAC and the Reference Variety aredescribed elsewhere herein and also can be seen in Tables 1 and 2. Whencomparing carrot variety NUN 85935 CAC to other varieties, thedistinguishing characteristics may be different. In one aspect, thedistinguishing characteristics may therefore include at least one, two,three or more (or all) of the characteristics listed in Tables 1 and 2.All numerical distinguishing characteristics are statisticallysignificantly different at p<0.05 between carrot variety NUN 85935 CACand the other variety (e.g., Reference Variety).

Carrot variety NUN 85935 CAC has the following distinguishingcharacteristics when compared to the Reference Variety as shown in Table3: 1) shorter plant top height; 2) thinner plant top neck diameter; 3)shorter blade length minus the petiole; 4) shorter petiole length; 5)thinner petiole diameter; 6) longer taproot length; 7) thinner rootshoulder diameter; 8) darker green leaf blade color; 9) more petiolepubescence; 10) smoother root surface; 11) prominent halo; 12) darkerand more reddish orange below ground root skin color; 13) strongerorange root cross section xylem color; and 14) darker reddish orangeroot cross section phloem color, where the characteristics of carrotvariety NUN 85935 CAC are compared to the characteristics of ReferenceVariety, when grown under the same environmental conditions.

Thus, a carrot plant “comprising the distinguishing characteristics ofcarrot variety NUN 85935 CAC” (such as a progeny plant) refers herein toa plant which does not differ significantly from said variety in thedistinguishing characteristics above. Therefore, in one aspect, thedisclosure provides a plant which does not differ significantly fromcarrot variety NUN 85935 CAC in the distinguishing characteristicsabove.

Similarity and differences between two different plant lines orvarieties can be determined by comparing the number of morphologicaland/or physiological characteristics that are the same (i.e.,statistically not significantly different) or that are different (i.e.,statistically significantly different) between the two plant lines orvarieties grown under the same environmental conditions. A numericalcharacteristic is considered to be “the same” when the value for anumeric characteristic is not significantly different at the 1% (p<0.01)or 5% (p<0.05) significance level, using one way Analysis of variance(ANOVA), a standard method known to the skilled person. Non-numerical or“degree” or “type” characteristic is considered “the same” when thevalues have the same “degree” or “type” when scored using USDA and/orUPOV descriptors, if the plants are grown under the same environmentalconditions.

As used herein, the term “variety”, “cultivated carrot” or “cultivar”means a plant grouping within a single botanical taxon of the lowestknown rank, which grouping, irrespective of whether the conditions forthe grant of a breeder's right are fully met, can be defined by theexpression of the characteristics resulting from a given genotype orcombination of genotypes, distinguished from any other plant grouping bythe expression of at least one of the said characteristics andconsidered as a unit with regard to its suitability for being propagatedunchanged.

A “plant line” is, for example, a breeding line which can be used todevelop one or more varieties. A breeding line is typically highlyhomozygous.

“Hybrid variety” or “F1 hybrid” refers to the seeds harvested fromcrossing two inbred (nearly homozygous) parental lines. For example, thefemale parent is pollinated with pollen of the male parent to producehybrid (F1) seeds on the female parent.

“Regeneration” refers to the development of a plant from cell culture ortissue culture or vegetative propagation.

“Vegetative propagation”, “vegetative reproduction” or “clonalpropagation” are used interchangeably herein and mean a method of takinga part of a plant and allowing that plant part to form at least roots,and also refer to the plant or plantlet obtained by that method.Optionally, the vegetative propagation is grown into a mature plant. Theskilled person is aware of what plant parts are suitable for use in themethod.

“Crossing” refers to the mating of two parent plants. The termencompasses “cross-pollination” and “selfing”.

“Selfing” refers to self-pollination of a plant, i.e., the transfer ofpollen from the anther to the stigma of the same plant.

“Cross-pollination” refers to the fertilization by the union of twogametes from different plants.

As used herein, the terms “resistance” and “tolerance” are usedinterchangeably to describe plants that show no symptoms orsignificantly reduced symptoms to a specified biotic pest, pathogen,abiotic influence or environmental condition compared to a susceptibleplant. These terms are optionally also used to describe plants showingsome symptoms but that are still able to produce marketable product withan acceptable yield.

The term “traditional breeding techniques” encompasses herein crossing,selfing, selection, doubled haploid production, embryo rescue,protoplast fusion, marker assisted selection, mutation breeding etc. asknown to the breeder (i.e., methods other than geneticmodification/transformation/transgenic methods), by which, for example,a genetically heritable trait can be transferred from one carrot line orvariety to another.

“Backcrossing” is a traditional breeding technique used to introduce atrait into a plant line or variety. The plant containing the trait iscalled the donor plant and the plant into which the trait is transferredis called the recurrent parent. An initial cross is made between thedonor parent and the recurrent parent to produce a progeny plant.Progeny plants which have the trait are then crossed to the recurrentparent. After several generations of backcrossing and/or selfing therecurrent parent comprises the trait of the donor. The plant generatedin this way may be referred to as a “single trait converted plant”. Thetechnique can also be used on a parental line of a hybrid.

“Progeny” as used herein refers to a plant obtained from a plantdesignated NUN 85935 CAC. A progeny may be obtained by regeneration ofcell culture or tissue culture or parts of a plant of said variety orselfing of a plant of said variety or by producing seeds of a plant ofsaid variety. In further aspects, progeny may also encompass plantsobtained from crossing of at least one plant of said variety withanother carrot plant of the same variety or another variety or(breeding) line, or with wild carrot plants. A progeny may comprise amutation or a transgene. A “first generation progeny” or is the progenydirectly derived from, obtained from, obtainable from or derivable fromthe parent plant by, e.g., traditional breeding methods (selfing and/orcross-pollinating) or regeneration (optionally combined withtransformation or mutation). Thus, a plant of carrot variety NUN 85935CAC is the male parent, the female parent or both of a first generationprogeny of carrot variety NUN 85935 CAC. Progeny may have all thephysiological and morphological characteristics of variety NUN 85935 CACwhen grown under the same environmental conditions. Using commonbreeding methods such as backcrossing or recurrent selection, mutationor transformation, one or more specific characteristics may beintroduced into said variety, to provide a plant comprising all but 1,2, or 3 or more of the morphological and physiological characteristicsof carrot variety NUN 85935 CAC.

The terms “gene converted” or “conversion plant” or “single locusconverted plant” in this context refer to carrot plants which aredeveloped by traditional breeding techniques, e.g., backcrossing or viagenetic engineering or through mutation breeding, wherein essentiallyall of the desired morphological and physiological characteristics ofthe parent variety or line are recovered, in addition to the one or morecharacteristics introduced into the parent transferred into the parentvia e.g., the backcrossing technique (optionally including reversebreeding or reverse synthesis of breeding lines). It is understood thatonly the addition of a further characteristics (e.g., addition of geneconferring a further characteristic, such as a disease resistance gene),but also the replacement/modification of an existing characteristics bya different characteristic is encompassed herein (e.g., mutant allele ofa gene can modify the phenotype of a characteristic).

Likewise, a “Single Locus Converted (Conversion) Plant” refers to plantsdeveloped by plant breeding techniques comprising or consisting ofmutation and/or by genetic transformation and/or by traditional breedingtechniques, such as backcrossing, wherein essentially all of the desiredmorphological and physiological characteristics of a carrot variety arerecovered in addition to the characteristics of the single locus havingbeen transferred into the variety via the backcrossing technique, orwherein the morphological and physiological characteristic of thevariety has been replaced/modified in the variety. In case of a hybrid,the gene may be introduced in the male or female parental line.

“Average” refers herein to the arithmetic mean.

The term “mean” refers to the arithmetic mean of several measurements.The skilled person understands that the appearance of a plant depends tosome extent on the growing conditions of said plant. Thus, the skilledperson understands suitable growing conditions for carrot variety NUN85935 CAC. The mean, if not indicated otherwise within this application,refers to the arithmetic mean of measurements on at least 10 different,randomly selected plants of a variety or line.

DETAILED DESCRIPTION OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure relates to a plant of carrot variety NUN 85935 CAC,wherein a representative sample of seeds of said carrot variety has beendeposited under the Budapest Treaty, with Accession number NCIMB ______.NUN 85935 CAC is an Imperator carrot variety for the cut and peel marketsegment and is suitable for the open field.

The disclosure relates to a seed of carrot variety NUN 85935 CAC,wherein a representative sample of said seed has been deposited underthe Budapest Treaty, with Accession number NCIMB ______.

In another aspect, the disclosure provides for a carrot plant part ofvariety NUN 85935 CAC, preferably a root, a representative sample ofseed from said variety has been deposited under the Budapest Treaty,with Accession number NCIMB ______.

A seed of hybrid carrot variety NUN 85935 CAC is obtainable by crossingthe male parent of carrot variety NUN 85935 CAC with the female parentof carrot variety NUN 85935 CAC and harvesting the seeds produced on thefemale parent. The resultant seeds of said variety can be grown toproduce plants of said variety. In one aspect, a seed or a plurality ofseeds of said variety are packaged into a container of any size or type(e.g., bags, cartons, cans, etc.). The seed may be disinfected, primedand/or treated with various compounds, such as seed coatings or cropprotection compounds. The seed produces a plant of carrot variety NUN85935 CAC.

Also provided is a plant of carrot variety NUN 85935 CAC, or a root orother plant part thereof, produced from a seed, wherein a representativesample of said seeds has been deposited under the Budapest Treaty, withAccession Number NCIMB ______.

Also provided is a plant part obtained from carrot variety NUN 85935CAC, wherein said plant part is: a root, or a part of a root, aharvested root, a root tip, a fruit, a harvested fruit, a part of afruit, a leaf, a part of a leaf, pollen, an ovule, a cell, a petiole, ashoot or a part thereof, a stem or a part thereof, a cutting, a seed, apart of a seed, seed coat or another maternal tissue which is part of aseed grown on said varieties, hypocotyl, cotyledon, a pistil, an anther,and a flower or a part thereof. Roots are particularly important plantparts. In a further aspect, the plant part obtained from carrot varietyNUN 85935 CAC is a cell, optionally a cell in a cell or tissue culture.That cell may be grown into a plant of carrot variety NUN 85935 CAC. Apart of carrot variety NUN 85935 CAC (or of progeny of that variety orof a plant having all physiological and/or morphological characteristicsbut one, two or three which are different from those of carrot varietyNUN 85935 CAC) further encompasses any cells, tissues, organs obtainablefrom the seedlings or plants in any stage of maturity.

The disclosure also provides for a food or feed product or a processedproduct comprising or consisting of a plant part described herein,wherein the plant part can be identified as a part of the plantdescribed herein. Preferably, the plant part is a carrot root or partthereof and/or an extract from a root or another plant part describedherein comprising at least one cell of carrot variety NUN 85935 CAC. Thefood or feed product may be fresh or processed, e.g., dried, grinded,powdered, pickled, chopped, cooked, roasted, in a sauce, in a sandwich,pasted, puréed or concentrated, juiced, pickled, canned, steamed,boiled, fried, blanched and/or frozen, baby-carrots etc.

Such a plant part of carrot variety NUN 85935 CAC can be stored and/orprocessed further. The disclosure also provides for a food or feedproducts comprising one or more of such parts, such as canned, chopped,cooked, roasted, in a sauce, in a sandwich, pasted, puréed orconcentrated, juiced, frozen, dried, pickled, or powdered carrot rootfrom carrot variety NUN 85935 CAC or from progeny of said variety, orfrom a derived variety, such as a plant having all but one, two or threephysiological and/or morphological characteristics of carrot variety NUN85935 CAC.

In another aspect, the disclosure provides for a carrot root of varietyNUN 85935 CAC, or a part of a root of said variety. The root can be inany stage of maturity, for example, immature or mature. In anotheraspect, the disclosure provides for a container comprising or consistingof a plurality of harvested carrot roots or parts of roots of saidvariety, or roots of progeny thereof, or roots of a derived variety.

In another aspect, the plant, plant part or seed of carrot variety NUN85935 CAC is inside one or more containers. For example, the disclosureprovides containers such as cans, boxes, crates, bags, cartons, ModifiedAtmosphere Packaging, films (e.g., biodegradable films), etc. comprisinga plant or a part of a plant (fresh and/or processed) or a seed ofcarrot variety NUN 85935 CAC. In a particular aspect, the containercomprises a plurality of seeds of carrot variety NUN 85935 CAC, or aplurality of plant parts of carrot variety NUN 85935 CAC.

The disclosure further relates to a carrot variety NUN 85935 CAC, whichwhen compared to its Reference Variety has the following distinguishingcharacteristics as shown in Table 3: 1) shorter plant top height; 2)thinner plant top neck diameter; 3) shorter blade length minus thepetiole; 4) shorter petiole length; 5) thinner petiole diameter; 6)longer taproot length; 7) thinner root shoulder diameter; 8) darkergreen leaf blade color; 9) more petiole pubescence; 10) smoother rootsurface; 11) prominent halo; 12) darker and more reddish orange belowground root skin color; 13) stronger orange root cross section xylemcolor; and 14) darker reddish orange root cross section phloem color,where the characteristics are determined at the 5% significance levelfor plants grown under the same environmental conditions. Alsoencompassed are parts of that plant.

In one aspect, a plant of carrot variety NUN 85935 CAC or a progenyplant thereof, comprises all of the following morphological and/orphysiological characteristics (i.e., average values of distinguishingcharacteristics, as indicated on the USDA Objective description ofvariety—carrot (unless indicated otherwise)) as shown in Tables 1 and2: 1) shorter plant top height; 2) thinner plant top neck diameter; 3)shorter blade length minus the petiole; 4) shorter petiole length; 5)thinner petiole diameter; 6) longer taproot length; 7) thinner rootshoulder diameter; 8) darker green leaf blade color; 9) more petiolepubescence; 10) smoother root surface; 11) prominent halo; 12) darkerand more reddish orange below ground root skin color; 13) strongerorange root cross section xylem color; and 14) darker reddish orangeroot cross section phloem color, where the characteristics aredetermined at the 5% significance level for plants grown under the sameenvironmental conditions. A part of this plant is provided.

The disclosure further provides a carrot plant which does not differfrom the physiological and morphological characteristics of the plant ofcarrot variety NUN 85935 CAC as determined at the 1%, 2%, 3%, 4% or 5%significance level when grown under the same environmental conditions.In a particular aspect, the plants are measured in the same trial (e.g.,the trial is conducted as recommended by the USDA or UPOV). Thedisclosure also comprises a part of said plant, preferably a root or apart thereof.

The disclosure also provides a tissue or cell culture comprising cellsof carrot variety NUN 85935 CAC. Such a tissue culture can, for example,be grown on plates or in liquid culture, or be frozen for long termstorage. The cells of carrot variety NUN 85935 CAC used to start theculture can be selected from any plant part suitable for vegetativereproduction, or in a particular aspect can be selected from an embryo,a meristem, a cotyledon, a hypocotyl, pollen, a leaf, an anther, a root,a root tip, a pistil, a petiole, a flower, a fruit, a seed, a stem and astalk. In another particular aspect, the tissue culture does not containsomaclonal variation or has reduced somaclonal variation. The skilledperson is familiar with methods to reduce or prevent somaclonalvariation, including regular reinitiation.

In one aspect, the disclosure provides a carrot plant regenerated fromthe tissue or cell culture of carrot variety NUN 85935 CAC, wherein theregenerated plant is not significantly different from carrot variety NUN85935 CAC in all, or all but one, two or three, of the physiological andmorphological characteristics (determined at the 5% significance levelwhen grown under the same environmental conditions). Optionally, theplant has one, two or three the physiological and morphologicalcharacteristics that are affected by a mutation or by transformation. Inanother aspect, the disclosure provides a carrot plant regenerated fromthe tissue or cell culture of carrot variety NUN 85935 CAC, wherein theplant has all of the physiological and morphological characteristics ofsaid variety determined (e.g., at the 5% significance level) when grownunder the same environmental conditions. Similarity or difference of acharacteristic is determined by measuring the characteristics of arepresentative number of plants grown under the same environmentalconditions, determining whether type/degree characteristics are the sameor different and determining whether numerical characteristics aredifferent at the 5% significance level.

Carrot variety NUN 85935 CAC, or its progeny, or a plant having allphysiological and/or morphological characteristics but one, two or threewhich are different from those of carrot variety NUN 85935 CAC, can alsobe reproduced using vegetative reproduction methods. Therefore, thedisclosure provides for a method of producing a plant, or a plant partof carrot variety NUN 85935 CAC, comprising vegetative propagation ofsaid variety. Vegetative propagation comprises regenerating a wholeplant from a plant part of carrot variety NUN 85935 CAC or from aprogeny or from or a plant having all physiological and/or morphologicalcharacteristics of said variety but one, two or three differentcharacteristics, such as a cutting, a cell culture or a tissue culture.

The disclosure also provides methods of vegetatively propagating a partof the plant of carrot variety NUN 85935 CAC. In certain aspects, themethod comprises: (a) collecting tissue or cells capable of beingpropagated from carrot variety NUN 85935 CAC to obtain proliferatedshoots; (b) rooting said proliferated shoots to obtain rooted plantlets.Steps (a) and (b) may also be reversed, i.e., first cultivating saidtissue to obtain roots and then cultivating the tissue to obtain shoots,thereby obtaining rooted plantlets. The rooted plantlets may then befurther grown, to obtain plants. In one aspect, the method furthercomprises step (c) growing plants from said rooted plantlets. Therefore,the method also comprises regenerating a whole plant from said part ofcarrot variety NUN 85935 CAC. In a particular aspect, the part of theplant to be propagated is a cutting, a cell culture or a tissue culture.

The disclosure also provides for a vegetatively propagated plant ofcarrot variety NUN 85935 CAC (or from progeny of said variety or from ora plant having all but one, two or three physiological and/ormorphological characteristics of carrot variety NUN 85935 CAC) whereinthe plant has all of the morphological and physiological characteristicsof carrot variety NUN 85935 CAC, when the characteristics are determinedat the 5% significance level for plants grown under the same conditions.In another aspect, the propagated plant has all but one, two or three ofthe morphological and physiological characteristics of carrot varietyNUN 85935 CAC, when the characteristics are determined at the 5%significance level for plants grown under the same conditions. A part ofsaid propagated plant or said propagated plant with one, two or threedifferences is also provided.

In another aspect, the disclosure provides a method for producing acarrot plant part, such as a root, comprising growing a plant of carrotvariety NUN 85935 CAC until it develops a root, and collecting the root.Preferably, the root is collected at harvest maturity. In anotheraspect, the root is collected at baby stage. A plant of carrot varietyNUN 85935 CAC can be produced by seeding directly in the soil (e.g.,field) (see, e.g., https://anrcatalog.ucanr.edu/pdf/7226.pdf).

In still another aspect, the disclosure provides a method of producing acarrot plant, comprising crossing a plant of carrot variety NUN 85935CAC with a second carrot plant at least once, allowing seed to developand optionally harvesting said respective progeny seed. The skilledperson can select progeny from said crossings. Optionally, therespective progeny is crossed twice, thrice, or four, five, six or seventimes, and allowed to set seed. In another aspect, the first step in“crossing” comprises planting seeds of a first and a second parentcarrot plant, often in proximity so that pollination will occur; forexample, mediated by insect vectors. Alternatively, pollen can betransferred manually. Where the plant is self-pollinated, pollinationmay occur without the need for direct human intervention other thanplant cultivation. After pollination the plant can produce seed.

In yet another aspect, the disclosure provides a method of producing aplant, comprising selfing a plant of carrot variety NUN 85935 CAC one ormore times, and selecting a progeny plant from said selfing. In oneaspect, the progeny plant retains all the distinguishing characteristicsof carrot variety NUN 85935 CAC described above, when grown under thesame environmental conditions. In a different aspect, the progeny plant,comprises all (or all but one, two or three) of the physiological andmorphological characteristic of carrot variety NUN 85935 CAC as listedin Tables 1 and 2.

In other aspects, the disclosure provides a progeny plant of carrotvariety NUN 85935 CAC, such as a progeny plant obtained by furtherbreeding that variety. Further breeding with carrot variety NUN 85935CAC, includes selfing that variety and/or cross-pollinating that varietywith another carrot plant or variety one or more times. In a particularaspect, the disclosure provides for a progeny plant that retains all orall but one, two, or three of the morphological and physiologicalcharacteristics of carrot variety NUN 85935 CAC, optionally all or allbut one, two, or three characteristics as listed in Tables 1 and 2,determined at the 5% significance level for numerical characteristics,when grown under the same environmental conditions. In another aspect,the progeny is a first generation progeny, i.e., the ovule or the pollen(or both) used in the crossing is an ovule or pollen of carrot varietyNUN 85935 CAC, i.e., the pollen comes from an anther of carrot varietyNUN 85935 CAC and the ovule comes from an ovary of carrot variety NUN85935 CAC. In another aspect, the disclosure provides for a vegetativereproduction of the variety and a plant having all, or all but one, two,or three of the physiological and morphological characteristics ofcarrot variety NUN 85935 CAC (e.g., as listed in Tables 1 and 2).

The disclosure also provides a method for collecting pollen of carrotvariety NUN 85935 CAC, comprising collecting the pollen from a plant ofcarrot variety NUN 85935 CAC. Alternatively, the method comprisesgrowing a plant of carrot variety NUN 85935 CAC until at least oneflower contains pollen and collecting the pollen. In a particularaspect, the pollen is collected when it is mature or ripe. A suitablemethod for collecting pollen comprises collecting anthers or the part ofthe anther that contains pollen, for example, by cutting it off. Pollencan be collected in containers. Optionally, collected pollen can be usedto pollinate a carrot flower.

The morphological and/or physiological differences between two differentindividual plants described herein (e.g., between carrot NUN 85935 CACand a progeny of said carrot variety) or between a plant of carrotvariety NUN 85935 CAC or progeny of said variety, or a plant having all,or all but 1, 2, or 3 of the physiological and morphologicalcharacteristics of carrot variety NUN 85935 CAC and another knownvariety can easily be established by growing said variety next to eachother or next to the other variety (in the same field, under the sameenvironmental conditions; in the same field, optionally, next to eachother), preferably in repeated several locations which are suitable forcultivation of carrots, and measuring morphological and/or physiologicalcharacteristics of a representative number of plants (e.g., to calculatean average value and to determine the variation range/uniformity withinthe variety). For example, trials can be carried out in Acampo Calif.,USA (N 38 degrees 07′261″/W 121 degrees 18′ 807″, USA), whereby variouscharacteristics, for example maturity, days from seeding to harvest,plant habit, leaf color, petiole anthocyanin, root shape, root collar,root halo, root shoulder, number of secondary root scars, diseaseresistance, insect resistance, can be measured and directly compared forspecies of carrot. Thus, the disclosure comprises carrot plant havingone, two or three physiological and/or morphological characteristicswhich are different from those of the plant of carrot variety NUN 85935CAC and which otherwise has all the physiological and morphologicalcharacteristics of the plant of carrot variety NUN 85935 CAC, whendetermined at the 5% significance level for plants grown under the sameenvironmental conditions. In another aspect, the differentcharacteristic is affected by a mutation, optionally induced mutation,or by transformation.

The morphological and physiological characteristics of carrot varietyNUN 85935 CAC are provided, for example, in Tables 1 and 2, as collectedin a trial according to USDA and/or UPOV standards. Encompassed hereinis also a plant obtainable from carrot variety NUN 85935 CAC (e.g., byselfing and/or crossing and/or backcrossing with said variety and/orprogeny of said variety) comprising all or all but one, two or three ofthe physiological and morphological characteristics of carrot varietyNUN 85935 CAC listed in Tables 1 and 2 (as determined at the 5%significance level for numerical characteristics or identical fornon-numerical characteristics) when grown under the same environmentalconditions and/or comprising one or more (or all; or all except one, twoor three) characteristics when grown under the same environmentalconditions. The morphological and/or physiological characteristics mayvary somewhat with variation in the environment (such as temperature,light intensity, day length, humidity, soil, fertilizer use, diseasevectors), which is why a comparison under the same environmentalconditions is preferred. Colors can best be measured using RoyalHorticultural Society (RHS) Chart.

Also, at-harvest and/or post-harvest characteristics of roots can becompared, such as by cold storage holding quality (browning),post-harvest rind firmness and/or flesh firmness, and juiciness can bemeasured using known methods.

The disclosure also provides for a method of producing a new carrotplant. The method comprises crossing a plant of carrot variety NUN 85935CAC, or a plant comprising all but 1, 2, or 3 of the morphological andphysiological characteristics of said variety (as listed in Tables 1 and2), or a progeny plant thereof, either as male or as female parent, witha second carrot plant (or a wild relative of carrot) one or more times,and/or selfing a carrot plant of variety NUN 85935 CAC, or a progenyplant thereof, one or more times, and selecting progeny from saidcrossing and/or selfing. The second carrot plant may, for example, be aline or variety of the species Daucus carota, or other Daucus species oreven other Apiaceae species.

The disclosure provides for methods of producing plants which retain allthe morphological and physiological characteristics of a plant describedherein. The disclosure provides also for methods of producing a plantcomprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of carrot variety NUN 85935 CAC (e.g., aslisted in Tables 1 and 2), but which are still genetically closelyrelated to said carrot variety. The relatedness can, for example, bedetermined by fingerprinting techniques (e.g., making use of isozymemarkers and/or molecular markers such as Single-nucleotide polymorphism(SNP) markers, amplified fragment length polymorphism (AFLP) markers,microsatellites, minisatellites, Random Amplified Polymorphic DNA (RAPD)markers, restriction fragment length polymorphism (RFLP) markers andothers). A plant is “closely related” to carrot variety NUN 85935 CAC ifits DNA fingerprint is at least 80%, 90%, 95% or 98% identical to thefingerprint of carrot variety NUN 85935 CAC. In a particular aspect,AFLP markers are used for DNA fingerprinting (see, e.g., Vos et al.1995, Nucleic Acid Research 23: 4407-4414). A closely related plant mayhave a Jaccard's Similarity index of at least about 0.8, preferably atleast about 0.9, 0.95, 0.98 or more (see, e.g., Shim and Jorgensen,Theor Appl Genet (2000) 101:227-233). The disclosure also provides aplant and a variety obtained or selected by applying these methods oncarrot variety NUN 85935 CAC. Such a plant may be produced bytraditional breeding techniques or mutation or transformation or inanother aspect, a plant may simply be identified and selected amongstplants of said variety, or progeny of said variety, e.g., by identifyinga variant within carrot variety NUN 85935 CAC, which variant differsfrom the variety described herein in one, two, or three of themorphological and/or physiological characteristics (e.g.,characteristics listed in Tables 1 and 2). In one aspect, the disclosureprovides a plant of carrot variety NUN 85935 CAC having a Jaccard'sSimilarity index with said variety of at least 0.8, e.g., at least 0.85,0.9, 0.95, 0.98 or even at least 0.99.

In some aspects, the disclosure provides a carrot plant comprisinggenomic DNA having at least 95%, 96%, 97%, 98% or 99% sequence identitycompared to the genomic DNA sequence of a plant of carrot variety NUN85935 CAC, as deposited under Accession Number NCIMB ______. In someaspects, the carrot plant further comprises all or all but 1, 2, or 3 ofthe morphological and physiological characteristics of carrot varietyNUN 85935 CAC (e.g., as listed in Tables 1 and 2). In other aspects, thecarrot plant is a hybrid derived from a seed or plant of carrot varietyNUN 85935 CAC.

For the purpose of this disclosure, the “sequence identity” ofnucleotide sequences, expressed as a percentage, refers to the number ofpositions in the two optimally aligned 25 sequences which have identicalresidues (×100) divided by the number of positions compared. A gap,i.e., a position in the pairwise alignment where a residue is present inone sequence but not in the other, is regarded as a position withnon-identical residues. A pairwise global sequence alignment of twonucleotide sequences is found by aligning the two sequences over theentire length according to the Needleman and Wunsch global alignmentalgorithm described in Needleman and Wunsch, 1970, J. Mol. Biol.48(3):443-53). A full implementation of the Needleman-Wunsch globalalignment algorithm is found in the needle program in The EuropeanMolecular Biology Open Software Suite (see, e.g., EMBOSS, Rice et al.,Trends in Genetics June 2000, vol. 16, No. 6. pp. 276-277).

The disclosure also provides methods for determining the identity ofparental lines described herein, in particular the identity of thefemale line. US2015/0126380, which is incorporated by reference, relatesto a non-destructive method for analyzing maternal DNA of a seed. Inthis method, the DNA is dislodged from the seed coat surface and can beused to collect information on the genome of the maternal parent of theseed. This method for analyzing maternal DNA of a seed comprises thesteps of contacting a seed with a fluid to dislodge DNA from the seedcoat surface, and analyzing the DNA thus dislodged from the seed coatsurface using methods known in the art. The skilled person is thus ableto determine whether a seed has grown on a plant of a plant carrotvariety NUN 85935 CAC, or is a progeny of said variety, because the seedcoat of the seed is a maternal tissue genetically identical to carrotvariety NUN 85935 CAC. In one aspect, the disclosure relates to a carrotseed coat comprising maternal tissue of carrot NUN 85935 CAC. In anotherparticular aspect, the disclosure provides a method of identifying thefemale parental line of carrot variety NUN 85935 CAC by analyzing theseed coat or another maternal tissue of said seed.

By crossing and/or selfing also (one or more) single traits may beintroduced into the carrot variety NUN 85935 CAC (e.g., usingbackcrossing breeding schemes), while retaining the remainingmorphological and physiological characteristics of said variety and/orwhile retaining one or more or all distinguishing characteristics. Asingle trait converted plant may thereby be produced. For example,disease resistance genes may be introduced, genes responsible for one ormore quality traits, yield, etc. Both single genes (e.g., dominant orrecessive) and one or more QTLs (quantitative trait loci) may betransferred into NUN 85935 CAC by breeding with said variety.

Any pest or disease resistance genes may be introduced into carrotvariety NUN 85935 CAC, progeny of said variety or into a plantcomprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of carrot variety NUN 85935 CAC (e.g., aslisted in Tables 1 and 2). Resistance to one or more of the followingdiseases or pests may be introduced into plants described herein:Alternaria Leaf Blight (Alternaria dauci), Aster Yellows (Macrostelesfasctfrons), Cavity Spot (Pythium sulcatum and P. violae), CercosporaBlight or Carrot Early Blight (Cerocospora carotae), Bacterial Blight(Xanthomonas carotae), Powdery Mildew (Erysiphe heraclei), Phytium RootDieback (Pythium spp.), Sclerotinia Decay or Watery Soft Rot(Sclerotinia spp.), Cottony Soft Rot (Sclerotinia sclerotiorum),Southern Blight (Sclerotium rolfsii), Bacterial Soft Rot (Erwiniacarotovora), Black Root Rot (Alternaria radicina), Gray Mold (Botrytisspp.), Sour Rot (Geothrichurn spp.), Root Knot Nematode (Nleloidogynespp.), Stubby Root Nematode (Trichodorus spp., and Paratrichodorusspp.), Needle Nematode (Longidorus africanus), Nutsedges Yellow (Cyperusesculentus), Nutsedges Purple (C. rotundus), Saltmarsh Catterpillars(Estigmene acrea), Cotton-melon Aphid (Aphis gossypii), and/orSilverleaf Whitefly (Bemisia argentifolii). Other resistances, againstpathogenic viruses (e.g., Motley Dwarf Virus, Carrot Thin Leaf Virus),fungi, bacteria, nematodes, insects or other pests may also beintroduced.

The disclosure also provides a method for developing a carrot plant in acarrot breeding program, using a carrot plant of variety NUN 85935 CAC,or its parts as a source of plant breeding material. Suitable plantbreeding techniques are recurrent selection, backcrossing, pedigreebreeding, mass selection, mutation breeding and/or genetic markerenhanced selection. In one aspect, the method comprises crossing carrotvariety NUN 85935 CAC or progeny of said variety, or a plant comprisingall but 1, 2, or 3 or more of the morphological and physiologicalcharacteristics of carrot variety NUN 85935 CAC (e.g., as listed inTables 1 and 2), with a different carrot plant, and wherein one or moreoffspring of the crossing are subject to one or more plant breedingtechniques: recurrent selection, backcrossing, pedigree breeding, massselection, mutation breeding and genetic marker enhanced selection (see,e.g., Stein and Nothnagel, (1995) Plant Breeding 114, 1-11). Forbreeding methods in general, see, e.g., Principles of Plant Genetics andBreeding, 2007, George Acquaah, Blackwell Publishing, ISBN-13:978-1-4051-3646-4.

The disclosure also provides a carrot plant comprising at least a firstset of the chromosomes of carrot variety NUN 85935 CAC, a sample of seedof said carrot variety has been deposited under Accession Number NCIMB______; optionally further comprising a single locus conversion, whereinsaid plant has essentially all of the morphological and physiologicalcharacteristics of the plant comprising at least a first set of thechromosomes of said variety. In another aspect, this single locusconversion confers a trait of yield, storage properties, color, malesterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, environmental stress tolerance, modifiedcarbohydrate metabolism or modified protein metabolism.

In one aspect, a plant of carrot variety NUN 85935 CAC may also bemutated (by e.g., irradiation, chemical mutagenesis, heat treatment,etc.) and mutated seeds or plants may be selected in order to change oneor more characteristics of said variety. Methods such as TILLING may beapplied to carrot populations in order to identify mutants. Similarly,carrot variety NUN 85935 CAC may be transformed and regenerated, wherebyone or more chimeric genes are introduced into the variety or into aplant comprising all but 1, 2, 3, or more of the morphological andphysiological characteristics (e.g., as listed in Tables 1 and 2).Transformation can be carried out using standard methods, such asAgrobacterium tumefaciens mediated transformation or biolistics,followed by selection of the transformed cells and regeneration intoplants. A desired trait (e.g., genes conferring pest or diseaseresistance, herbicide, fungicide or insecticide tolerance, etc.) can beintroduced into carrot variety NUN 85935 CAC, or progeny of saidvariety, by transforming said variety or progeny of said variety with atransgene that confers the desired trait, wherein the transformed plantretains all or all but one, two or three of the physiological and/ormorphological and/or physiological characteristics of carrot variety NUN85935 CAC or the progeny of said variety and contains the desired trait.In another aspect, the transformation or mutation confers a traitwherein the trait is yield, storage properties, color, male sterility,herbicide tolerance, insect resistance, pest resistance, diseaseresistance, environmental stress tolerance, modified carbohydratemetabolism or modified protein metabolism.

The disclosure also provides a method of producing a carrot plant havinga desired trait, comprising mutating a plant of carrot variety NUN 85935CAC or a cell thereof and selecting a plant with the desired trait,wherein the mutated plant retains all or all but one of the phenotypicand morphological characteristics of said carrot variety, optionally asdescribed in Tables 1 and 2, and contains the desired trait and whereina representative sample of seed of carrot variety NUN 85935 CAC has beendeposited under Accession Number NCIMB ______. In a further aspect, thedesired trait is yield, high anthocyanin, root size and shape, storageproperties, male sterility, herbicide tolerance, insect resistance, pestresistance, disease resistance, environmental stress tolerance, modifiedcarbohydrate metabolism, modified protein metabolism and/or ripening.

A suitable method for inducing mutation in carrot variety NUN 85935 CACcomprises the steps of:

-   -   a. exposing the seed, the plant or the plant part or the cell of        carrot variety NUN 85935 CAC to a mutagenic compound or to        radiation, wherein a representative sample of seed of carrot        variety NUN 85935 CAC is deposited under Accession Number NCIMB        ______;    -   b. selecting the seed, the plant or the plant part or the cell        of carrot variety NUN 85935 CAC having a mutation; and    -   c. optionally growing and/or multiplying the seed, plant or        plant part or cell of NUN 85935 CAC having the mutation.

The disclosure also provides a plant having one, two or threephysiological and/or morphological characteristics which are differentfrom those of carrot variety NUN 85935 CAC and which otherwise has allthe physiological and morphological characteristics of said carrotvariety, wherein a representative sample of seed of carrot variety NUN85935 CAC has been deposited under Accession Number NCIMB ______. Inparticular, variants which differ from carrot variety NUN 85935 CAC innone, one, two or three of the characteristics mentioned in Tables 1 and2 are encompassed.

A part of carrot variety NUN 85935 CAC (or of progeny of said variety orof a plant having all physiological and/or morphological characteristicsbut one, two or three which are different from those of said variety)encompasses any cells, tissues, organs obtainable from the seedlings orplants, such as but not limited to: a carrot root or a part thereof, acutting, hypocotyl, cotyledon, seed coat, pollen and the like. Suchparts can be stored and/or processed further. Encompassed are thereforealso food or feed products comprising a part of carrot variety NUN 85935CAC or a part of progeny of said variety, or a part of a plant havingall but one, two or three physiological and/or morphologicalcharacteristics of carrot variety NUN 85935 CAC, comprising one or moreof such parts, optionally processed (such as canned, chopped, cooked,roasted, in a sauce, in a sandwich, pasted, puréed or concentrated,juiced, frozen, dried, pickled, or powdered).

In one aspect, the disclosure provides a haploid plant and/or a doubledhaploid plant of carrot variety NUN 85935 CAC, or of a plant having allbut one, two or three physiological and/or morphological characteristicsof carrot variety NUN 85935 CAC, or progeny of said carrot variety.Haploid and doubled haploid (DH) plants can, for example, be produced bycell or tissue culture and chromosome doubling agents and regenerationinto a whole plant. DH production chromosome doubling may be inducedusing known methods, such as colchicine treatment or the like. In oneaspect, the method comprises inducing a cell or tissue culture withchromosome doubling agent and regenerating the cells or tissues into awhole plant.

In another aspect, the disclosure comprises method for making doublehaploid cells from haploid cells of carrot variety NUN 85935 CAC,comprising doubling cells of carrot variety NUN 85935 CAC with achromosome doubling agent such as colchicine treatment (see, e.g.,Nikolova and Niemirowicz-Szczytt (1996) Acta Soc Bot Pol 65:311-317).

In another aspect, the disclosure provides haploid plants and/or doubledhaploid plants derived from carrot variety NUN 85935 CAC that, whencombined, make a set of parents of carrot variety NUN 85935 CAC. Thehaploid plant and/or the doubled haploid plant of carrot variety NUN85935 CAC can be used in a method for generating parental lines ofcarrot variety NUN 85935 CAC.

Using methods known in the art like “reverse synthesis of breedinglines” or “reverse breeding,” it is possible to produce parental linesfor a hybrid plant such as carrot variety NUN 85935 CAC. A skilledperson can take any individual heterozygous plant (called a“phenotypically superior plant” in Example 2 of US2015/0245570; which ishereby incorporated by reference in its entirety; carrot variety NUN85935 CAC is such a plant) and generate a combination of parental lines(reverse breeding parental lines) that, when crossed, produce thevariety NUN 85935 CAC. It is not necessary that the reverse breedingparental lines are identical to the original parental lines. Such newbreeding methods are based on the segregation of individual alleles inthe spores produced by a desired plant and/or in the progeny derivedfrom the self-pollination of that desired plant, and on the subsequentidentification of suitable progeny plants in one generation, or in alimited number of inbred cycles. Such a method is known fromUS2015/0245570 or from Wijnker et al., Nature Protocols Volume: 9,Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049. Thus, thedisclosure provides a method for producing parental lines for a hybridorganism (e.g., carrot variety NUN 85935 CAC), comprises in one aspect:a) defining a set of genetic markers that are present in a heterozygousform (H) in a partially heterozygous starting organism; b) producingdoubled haploid lines from spores of the starting organism: c)genetically characterizing the doubled haploid lines thus obtained forthe said set of genetic markers to determine whether they are present ina first homozygous form (A) or in a second homozygous form (B); and d)selecting at least one pair of doubled haploid lines that havecomplementary alleles for at least a subset of the genetic markers,wherein each member of the pair is suitable as a parental line for ahybrid organism.

In another aspect, the method for producing parental lines for hybridorganisms, e.g., of carrot variety NUN 85935 CAC, which when crossedreconstitute the genome of carrot variety NUN 85935 CAC, comprising:

-   -   a. defining a set genetic markers that are present a        heterozygous form (H) in a partially heterozygous starting        organism;    -   b. producing at least one further generation from the starting        organism by self-pollination (e.g., F2 or F3 generation);    -   c. selecting at least one pair of progeny organisms in which at        least one genetic marker from the set is present in a        complementary homozygous forms (B vs. A, or A vs. B); and    -   d. optionally repeating steps b) and c) until at least one pair        of progeny organisms that have complementary alleles for at        least a subset of the genetic markers had been selected as        parental lines for a hybrid.

The disclosure relates to a method of producing a combination ofparental lines of a plant of carrot variety NUN 85935 CAC, comprisingthe step of making doubled haploid cells from haploid cells from saidplant or a seed of that plant; and optionally crossing these parentallines to produce and collect seeds. In another aspect, the disclosurerelates to a combination of parental lines produced by this method. Instill another aspect, the combination of parental lines can be used toproduce a seed or plant of carrot variety NUN 85935 CAC when theseparental lines are crossed. In still another aspect, the disclosurerelates to a combination of parental lines from which a seed or planthaving all physiological and/or morphological characteristics of carrotvariety NUN 85935 CAC (when the characteristics are determined at the 5%significance level for plants grown under the same conditions).

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto carrot variety NUN 85935 CAC comprising:

-   -   a. obtaining a combination of a parental lines of carrot variety        NUN 85935 CAC, optionally through reverse synthesis of breeding        lines;    -   b. introducing a single locus conversion in at least one of the        parents of step a; and    -   c. crossing the converted parent with the other parent of step a        to obtain seed of carrot variety NUN 85935 CAC.

A combination of a male and a female parental line of carrot variety NUN85935 CAC can be generated by methods described herein, for example,through reverse synthesis of breeding lines.

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto carrot variety NUN 85935 CAC, comprising introducing a single locusconversion in at least one of the parents of carrot variety NUN 85935CAC, and crossing the converted parent with the other parent of carrotvariety NUN 85935 CAC to obtain seed of said carrot variety.

In another aspect, introducing a single locus conversion in at least oneof the parents comprise:

-   -   a. obtaining a cell or tissue culture of cells of the parental        line of carrot variety NUN 85935 CAC;    -   b. genetically transforming or mutating said cells;    -   c. growing the cells into a plant; and    -   d. optionally selecting plants that contain the single locus        conversion, the single trait conversion or the desired trait.

In another method, the step of introducing a single locus conversion,single trait conversion, or desired trait in at least one of the parentplants comprises:

-   -   a. crossing the parental line of carrot variety NUN 85935 CAC        with a second carrot plant comprising the single locus        conversion, the single trait conversion or the desired trait;    -   b. selecting F1 progeny plants that contain the single locus        conversion, the single trait conversion or the desired trait;    -   c. crossing said selected progeny plants of step b. with the        parental line of step a, to produce a backcross progeny plant;    -   d. selecting backcross progeny plants comprising the single        locus conversion, the single trait conversion or the desired        trait and otherwise all or all but one, two or three of the        morphological and physiological characteristics the parental        line of step a. to produce selected backcross progeny plants;        and    -   e. optionally repeating steps c. and d. one or more times in        succession to produce selected second, third or fourth or higher        backcross progeny plants comprising the single locus conversion,        the single trait conversion or the desired trait and otherwise        all or all but one, two or three of the morphological and        physiological characteristics the parental line of step a. to        produce selected backcross progeny plants, when grown in the        same environmental conditions.        The disclosure further relates to plants obtained by this        method.

In any of the above methods, wherein the single locus conversionconcerns a trait, the trait may be yield or pest resistance or diseaseresistance. In one aspect, the trait is disease resistance and theresistance is conferred to Alternaria Leaf Blight (Alternaria dauci),Aster Yellows (Macrosteles fascifrons), Cavity Spot (Pythium sulcatumand P. violae), Cercospora Blight or Carrot Early Blight (Cerocosporacarotae), Bacterial Blight (Xanthomonas carotae), Powdery Mildew(Erysiphe heraclei), Phytium Root Dieback (Pythium spp.), SclerotiniaDecay or Watery Soft Rot (Sclerotinia spp.), Cottony Soft Rot(Sclerotinia sclerotiorum), Southern Blight (Sclerotium rolfsii),Bacterial Soft Rot (Erwinia carotovora), Black Root Rot (Alternariaradicina), Gray Mold (Botrytis spp.), Sour Rot (Geothrichurn spp.), RootKnot Nematode (Meloidogyne spp.), Stubby Root Nematode (Trichodorusspp., and Paratrichodorus spp.), Needle Nematode (Longidorus africanus),Nutsedges Yellow (Cyperus esculentus), Nutsedges Purple (C. rotundus),Saltmarsh Catterpillars (Estigmene acrea), Cotton-melon Aphid (Aphisgossypii), and/or Silverleaf Whitefly (Bemisia argentifolii). Otherresistances, against pathogenic viruses (e.g., Motley Dwarf Virus,Carrot Thin Leaf Virus), fungi, bacteria, nematodes, insects or otherpests may also be introduced.

The disclosure also provides a combination of parental lines which, whencrossed, produce a seed or plant having all physiological and/ormorphological characteristics of carrot variety NUN 85935 CAC but one,two or three which are different (when grown under the sameenvironmental conditions), as well as a seed or plant having allphysiological and/or morphological characteristics of carrot variety NUN85935 CAC but one, two or three which are different (when thecharacteristics are determined at the 5% significance level for plantsgrown under the same conditions).

Also provided is a plant part obtainable from carrot variety NUN 85935CAC or from progeny of said variety or from a plant having all but one,two or three physiological and/or morphological characteristics whichare different from those of carrot variety NUN 85935 CAC, or from avegetatively propagated plant of carrot variety NUN 85935 CAC (or fromits progeny or from a plant having all or all but one, two or threephysiological and/or morphological characteristics which are differentfrom those of carrot variety NUN 85935 CAC), wherein said plant part isa root, or a part of a root, a harvested root, a root tip, a fruit, aharvested fruit, a part of a fruit, a leaf, a part of a leaf, pollen, anovule, a cell, a petiole, a shoot or a part thereof, a stem or a partthereof, a cutting, a seed, a part of a seed, seed-coat or anothermaternal tissue which is part of a seed grown on NUN 85935 CAC, orhypocotyl, cotyledon, a pistil, an anther, or a flower or a partthereof.

In another aspect, the disclosure provides a method of determining thegenotype of a plant of the disclosure comprising the step of detectingin the genome (e.g., a sample of nucleic acids) of the plant at least afirst polymorphism or an allele. The skilled person is familiar withmany suitable methods of genotyping, detecting a polymorphism ordetecting an allele including restriction fragment length polymorphismidentification (RFLP) of genomic DNA, random amplified polymorphicdetection (RAPD) of genomic DNA, amplified fragment length polymorphismdetection (AFLP), polymerase chain reaction (PCR), DNA sequencing,allele specific oligonucleotide (ASO) probes, and hybridization to DNAmicroarrays or beads. Alternatively, the entire genome could besequenced. The method may, in certain embodiments, comprise detecting aplurality of polymorphisms in the genome of the plant, for example byobtaining a sample of nucleic acid from a plant and detecting in saidnucleic acids a plurality of polymorphisms. The method may furthercomprise storing the results of the step of detecting the plurality ofpolymorphisms on a computer readable medium.

The disclosure further provides for a food or feed product comprising orconsisting a plant part of carrot variety NUN 85935 CAC or a part ofprogeny of said carrot variety, or a part of a plant having all but one,two, or three of the physiological and/or morphological characteristicsof carrot variety NUN 85935 CAC, comprising one or more such parts,optionally processed (such as canned, chopped, cooked, roasted, in asauce, in a sandwich, pasted, puréed or concentrated, juiced, frozen,dried, pickled, or powdered).

All documents (e.g., patent publications) are herein incorporated byreference in their entirety, including the following cited references:

-   UPOV, “Guidelines for the Conduct of Tests for Distinctness,    Uniformity and Stability, TG/49/8 (Geneva 2007, last updated in    2015-03-25), world-wide web at upov.int under    edocs/tgdocs/en/tg049.pdf.-   US Department of Agriculture, Agricultural Marketing Service,    “Objective description of Variety—Carrot (Daucus carota),” world    wide web at    ams.usda.gov/services/plant-variety-protection/pvpo-c-forms, under    carrot.-   Acquaah, G., “Principles of Plant Genetics and Breeding”, Blackwell    Publishing, 2007, ISBN-13: 978-1-4051-3646-4.-   Arnhold-Schmitt, B., et. al., “Physiological Aspects of Genome    Variability in Tissue Culture. I. Growth Phase-Dependent    Differential DNA Methylation of the Carrot Genome (Daucus carota L.)    During Primary Culture”, Theoretical and Applied Genetics, 1995,    vol. 91, no. 5, pp. 809-815-   Jhang, T., et. al., “Efficiency of Different Marker Systems for    Molecular Characterization of Subtropical Carrot Germplasm,” The    Journal of Agricultural Science, 2010, vol. 148, no. 2, pp. 171-181.-   Larkin, P. J., et. al., “Somaclonal Variation—A Novel Source of    Variability from Cell Cultures for Plant Improvement”, Theoretical    and Applied Genetics, 1981, vol. 60, no. 4, pp. 197-214.-   Martin, E., et al., “Identification of Markers Linked to Agronomic    Traits in Globe Artichoke”, Australian Journal of Crop Science,    2008, vol. 1(2), pp. 43-46.-   Needleman, S. B., et. al., “A General Method Applicable to the    Search for Similarities in the Amino Acid Sequence of Two Proteins”,    Journal of Molecular Biology, 1970, vol. 48(3), pp. 443-53.-   Nikolova, V., et. al., “Diploidization of Cucumber (Cucumis sativus    L.) Haploids by Colchini Treatment”, Acta Societas Botanicorum    Poloniae, 1996, vol. 65, pp. 311-317.-   Rice, P., et al., “EMBOSS: The European Molecular Biology Open    Software Suite”, Trends in Genetics, 2000, vol. 16, Issue 6. pp.    276-277.-   Shim, S. J., and Jorgensen, R. B., “Genetic Structure in Cultivated    and Wild Carrots (Daucus carota L.) Revealed by AFLP Analysis”,    Theor Appl Genet, 2000, vol. 101, pp. 227-233.-   Stein, M., et. al., “Some Remarks on Carrot Breeding (Daucus carota    saativus Hoffm.), Plant Breeding, 1995, vol. 114, no. 1, pp. 1-11.-   Vos, P., et al., “AFLP: A New Technique for DNA Fingerprinting”,    Nucleic Acids Research, 1995, vol. 23(21), pp. 4407-4414.-   Wijnker, E., et al., “Hybrid Recreation by Reverse breeding in    Arabidopsis thaliana”, Nature Protocols, 2014, vol. 9, pp. 761-772.    DOI: doi: 10.1038/nprot.2014.049-   US2015/0126380-   US2015/0245570-   https://www.ams.usda.gov/sites/default/files/media/19-Carrot    %2OST-470-19%202015.pdf-   https://anrcatalog.ucanr.edu/pdf/7226.pdf-   https://www.upov.int/edocs/tgdocs/en/tg049.pdf-   https://www.worldatlas.com/articles/the-world-s-top-carrot-producing-countries.html

Development of NUN 85935 CAC

The hybrid carrot variety NUN 85935 CAC was developed from a male andfemale proprietary inbred line of Nunhems. The female and male parentswere crossed to produce hybrid (F1) seeds of carrot variety NUN 85935CAC. The seeds of carrot variety NUN 85935 CAC can be grown to producehybrid plants and parts thereof (e.g., carrot roots). The hybrid carrotvariety NUN 85935 CAC can be propagated by seeds or vegetative.

The hybrid variety is uniform and genetically stable. This has beenestablished through evaluation of horticultural characteristics. Severalhybrid seed production events resulted in no observable deviation ingenetic stability. Coupled with the confirmation of genetic stability ofthe female and male parents the Applicant has concluded that carrotvariety NUN 85935 CAC is uniform and stable.

Deposit Information

A total of 2500 seeds of the hybrid carrot variety NUN 85935 CAC will bedeposited according to the Budapest Treaty by Nunhems B.V. on ______, atthe NCIMB Ltd., Ferguson Building, Craibstone Estate, Bucksburn,Aberdeen AB21 9YA, United Kingdom (NCIMB). The deposit will be assignedNCIMB number ______. A deposit of carrot variety NUN 85935 CAC and ofthe male and female parent line is also maintained at Nunhems B.V.

Access to the deposits will be available during the pendency of thisapplication to persons determined by the Director of the U.S. PatentOffice to be entitled thereto upon request. Subject to 37 C.F.R. §1.808(b), all restrictions imposed by the depositor on the availabilityto the public of the deposited material will be irrevocably removed uponthe granting of the patent. The deposit will be maintained for a periodof 30 years, or 5 years after the most recent request, or for theenforceable life of the patent whichever is longer and will be replacedif it ever becomes nonviable during that period. Applicant does notwaive any rights granted under this patent on this application or underthe Plant Variety Protection Act (7 U.S.C. § 2321 et seq.).

Characteristics of Carrot Variety NUN 85935 CAC

The most similar variety to carrot variety NUN 85935 CAC is a varietyfrom Seminis with the commercial name PS 1441.

In Tables 1 and 2, a comparison between carrot variety NUN 85935 CAC andthe Reference Variety (PS 1441) is shown based on a trial in the USAduring the trial season 2018. Trial location: California, US; Harvestdate: Feb. 15, 2018. In Table 3, the distinguishing characteristicsbetween carrot variety NUN 85935 CAC and the Reference Variety (PS 1441)is shown.

A trial of 30 plants of each variety, from which at least 15 plants orplant parts were randomly selected and were used to measurecharacteristics. For numerical characteristics, averages werecalculated. For non-numerical characteristics, the type/degree weredetermined.

In one aspect, the disclosure provides a plant having the physiologicaland morphological characteristics of carrot variety NUN 85935 CAC aspresented in Tables 1 and 2.

TABLE 1 Objective Description of Carrot Variety NUN 85935 CAC and theReference Variety (USDA Descriptors); where quantitative values arementioned these are statistically significantly different between CarrotVariety NUN 85935 CAC and the Reference Variety using an ANOVA Tukeytest. USDA Descriptors NUN 85935 CAC PS 1441 Type: 1 = Amsterdam; 2 =Flakee; 3 = Cut and peel Cut and peel Berlicum; 4 = Chantenay; 5 =Danvers; 6 = Imperator; 7 = Nantes; 8 = Other (Specify) Region ofAdaptation in the U.S.A.: 1 = Northeast; 2 = Northwest; 3 = Most regionsMost regions Southeast; 4 = Southwest; 5 = North Central; 6 = SouthCentral; 7 = Most regions Market Maturity: No. of Days from Seeding toHarvest 120    120    Plant Top (at harvest stage): Habit: 1 = Erect; 2= Semi-erect; 3 = Semi-erect Semi-erect Prostrate Plant Top Height (fromShoulder to 42.16 47.75 Top of Crown), cm Plant Top Neck Diameter, mm 9.76 11.08 Top Attachment: 1 = Single; 2 = Single Single Multiple Leaf(at harvest stage): Blade Color: 1 = Light Green; 2 = Medium greenMedium green Medium Green; 3 = Dark Green; 4 = (RHS 137C) (RHS 147B)Other (Specify) Blade Divisions: 1 = Fine; 2 = Medium Medium Medium; 3 =Coarse Blade Length (Without Petiole), cm 17.75 23.32 Petiole Lengthfrom Crown to First 21.33 23.65 Pinna, cm Petiole Anthocyanin: 1 =Absent; 2 = Absent Absent Present Petiole Pubescence: 1 = Absent; 2 =Present Absent Present Root (at market maturity): Cortex Thickness(Midpoint X-  5.14  5.41 Section), mm Core Thickness (MidpointX-Section),  4.40  3.97 mm Carrot Length (Minus Taproot), cm 30.8  28.7 Length of Taproot, mm 44.95 31.76 Diameter at Shoulder, mm 15.78 18.93Diameter at Midpoint, mm 16.22 15.56 Amount Exposed (Above Ground): NoneNone 1 = None; 2 = 1-10%; 3 = 11-20%; 4 = 21-30%; 5 = 31-40%; 6 = >40%Shape: 1 = Round; 2 = Conic; 3 = Cylindrical Cylindrical CylindricalCollar: 1 = Sunken; 2 = Level; 3 = Level Level Square Shoulder: 1 =Rounded; 2 = Sloping; Rounded Rounded 3 = Square Base: 1 = Pointed; 2 =Medium; 3 = Pointed Pointed Blunt Surface Smoothness: Very smoothDimpled or 1 = Very Smooth; 2 = Dimpled or corrugated Corrugated Numberof Secondary Root Scars: Few Few 1 = None; 2 = Few; 3 = Many Appearanceof Secondary Root Scars: Not prominent Not prominent 1 = Not Prominent;2 = Prominent Halo: 1 = None; 2 = Faint; 3 = Prominent Faint ProminentZoning: 1 = None; 2 = Faint; 3 = Prominent Prominent Prominent Colors(RHS Colour Chart): Color choices: 1 = white; 2 = yellow; 3 = orange; 4= red; 5 = green; 7 = salmon; 8 = light; 9 = dark; 10 = other; colorexamples: 02 = yellow; 34 = orange-red; 94 = dark red Below GroundExterior Color: Light orange Light orange Shoulder (RHS N163A) (RHSN163A) Below Ground Exterior Color: Skin Dark orange Orange (RHS N172B)(RHS 170B) X-Section Interior Color: Core Dark orange Dark orange(N172C) (RHS 170B) X-Section Interior Color: Phloem Orange Orange (RHSN172B) (RHS 172C)

TABLE 2 Objective Description of Carrot Variety NUN 85935 CAC and theReference Variety (Non-USDA Descriptors); where quantitative values arementioned these are statistically significantly different between CarrotVariety NUN 85935 CAC and the Reference Variety using an ANOVA Tukeytest. Non - USDA Descriptors NUN 85935 CAC PS 1441 Petiole Diameter, mm2.66 3.13 Root Weight, grams 45.33 56.93 Brix (sugar content) 10.77°11.39°

TABLE 3 Distinguishing Characteristics between Carrot Variety NUN 85935CAC and Reference Variety PS 1441 Characteristics NUN 85935 CAC PS 1441Plant top (at harvest stage): Plant Top Height (from Shoulder to 42.1647.75 Top of Crown), cm Plant Top Neck Diameter, mm  9.76 11.08 Leaf (atharvest stage): Blade Length (Without Petiole), cm 17.75 23.32 BladeColor: 1 = Light Green; 2 = Medium green Medium green Medium Green; 3 =Dark Green; 4 = (RHS 137C) (RHS 147B) Other (Specify) Petiole Lengthfrom Crown to First 21.33 23.65 Pinna, cm Petiole Diameter, mm  2.66 3.13 Petiole Pubescence: 1 = Absent; 2 = Present Absent Present Root(at market maturity): Length of Taproot, mm 44.95 31.76 Diameter atShoulder, mm 15.78 18.93 Surface Smoothness: Very smooth Dimpled or 1 =Very Smooth; 2 = Dimpled or corrugated Corrugated Halo: 1 = None; 2 =Faint; 3 = Prominent Faint Prominent Below Ground Exterior Color: SkinDark orange Orange (RHS N172B) (RHS 170B) X-Section Interior Color: CoreDark orange Dark orange (N172C) (RHS 170B) X-Section Interior Color:Phloem Orange Orange (RHS N172B) (RHS 172C)

1. A plant, plant part, or seed of carrot variety NUN 85935 CAC, wherein a representative sample of seed of said carrot variety is deposited under Accession Number NCIMB ______.
 2. A plant part of claim 1, wherein the plant part is a leaf, pollen, an ovule, a fruit, a root, a taproot, cutting, flower or a cell.
 3. A plant or a part thereof, produced by growing the seed of claim
 1. 4. A carrot plant or a part thereof having all of the physiological and morphological characteristics of the carrot plant of claim
 1. 5. A tissue or cell culture of regenerable cells of the plant or plant part of claim
 1. 6. The tissue or cell culture according to claim 5, comprising cells or protoplasts from a plant part suitable for vegetative reproduction, wherein the plant part is an embryo of carrot variety NUN 85935 CAC, a meristem, a cotyledon, a hypocotyl, a seed coat, a leaf, an anther, a root, a root tip, a taproot, a pistil, a petiole, a flower, a fruit, a seed of carrot variety NUN 85935 CAC, a stem, or a stalk.
 7. A carrot plant regenerated from the tissue or cell culture of claim 5, wherein the plant has all of the physiological and morphological characteristics of the carrot variety NUN 85935 CAC, when the numerical characteristics are determined at the 5% significance level for plants grown under the same environmental conditions, and wherein a representative sample of seed of carrot variety has been deposited under Accession Number NCIMB ______.
 8. A method of producing the plant of claim 1 or a part thereof, said method comprising vegetative propagation of at least a part of carrot variety NUN 85935 CAC.
 9. The method of claim 8, wherein said vegetative propagation comprises regenerating a whole plant from said part of carrot variety NUN 85935 CAC, wherein a representative sample of seed of said carrot variety has been deposited under Accession Number NCIMB ______.
 10. The method of claim 8, wherein said part is a cutting, a cell culture or a tissue culture.
 11. A vegetative propagated plant of claim 1, or a part thereof, wherein the vegetative propagated plant has all of the physiological and morphological characteristics of the plant of carrot variety NUN 85935 CAC, when the numerical characteristics are determined at the 5% significance level for plants grown under the same environmental conditions, and wherein a representative sample of seed of carrot variety has been deposited under Accession Number NCIMB ______.
 12. A method of producing a carrot plant, comprising crossing the plant of claim 1 with a second carrot plant at least once, selecting a progeny carrot plant from said crossing, and optionally allowing the progeny carrot plant to form seed, and wherein a representative sample of seed of carrot variety NUN 85935 CAC has been deposited under Accession Number NCIMB ______.
 13. A carrot plant having one physiological or morphological characteristic which is different from those of the plant of claim 1, and which otherwise has all the physiological and morphological characteristics of the plant of carrot variety NUN 85935 CAC, when the numerical characteristics are determined at the 5% significance level for plants grown under the same environmental conditions, and wherein a representative sample of seed of said carrot variety has been deposited under Accession Number NCIMB ______.
 14. A plant of carrot variety NUN 85935 CAC, further comprising a single locus conversion, wherein said plant has all of the morphological and physiological characteristics of the plant of claim 1, wherein the single locus conversion is introduced by genetic transformation, and wherein a representative sample of seed of said carrot variety has been deposited under Accession Number NCIMB ______, and wherein the single locus conversion confers male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, modified protein metabolism, or ripening.
 15. A method of producing doubled haploids of carrot variety NUN 85935 CAC comprising making double haploid cells from haploid cells made from the plant or the seed of claim 1 by chromosome doubling, and wherein a representative sample of seed of said carrot variety has been deposited under Accession Number NCIMB ______.
 16. A method of producing a carrot root comprising obtaining a plant according to claim 1, wherein the plant has been cultivated to maturity, and collecting the root from the plant.
 17. A container comprising the carrot root collected in the method of claim
 16. 18. A container comprising the plant part of claim 2, wherein the plant part is a carrot root or part thereof.
 19. A food or a feed product comprising the plant part of claim 2, wherein said plant part is a carrot root or parts thereof.
 20. A method of producing a carrot plant having a desired trait, wherein the method comprises mutating a carrot plant of variety NUN 85935 CAC and selecting a mutated plant with a desired trait, wherein the mutated plant retains all of the physiological and morphological characteristics of carrot variety NUN 85935 CAC, when grown under the same environmental conditions and contains the desired trait, wherein a representative sample of seed of said carrot variety has been deposited under Accession Number NCIMB ______, and wherein the desired trait is male sterility, herbicide tolerance, pest resistance, environmental stress resistance, modified carbohydrate metabolism, modified protein metabolism, or ripening. 